Rivers Angels, holders of the Women's President Federation Cup, face Abia Angels in Round of 16 action. The match takes place at Uyo Township Stadium in Eket on Tuesday, June 2.
FC Robo Queens will travel to Akure to battle Remo Stars Ladies. Ahudiyannem Queens host Heartland Queens at an Enugu venue.
Ibom Angels square off against Bayelsa Queens in Awka. Delta Queens meet Edo Queens in the same city.
Plateau United Queens face Sunshine Queens in Abuja on the same day. Confluence Queens take on Esthington FC in Enugu.
Naira Ratels complete the fixture list against 2025 runners-up Nasarawa Amazons. That match will be played in Kaduna.
